IGEM:UNAM-Genomics Mexico/2009/Notebook/H2 Pae/2011/07/13

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Results of yesterday's digestion

The following electrophoresis gel shows the results of the pBBR1MCS-5 double digestion by apaI and sacI

Lanes:

1. Ladder 10 kb
2. pBBR1MCS-5
3. Double digestion

Pae 13 jul 2011.JPG

This clearly shows that the plasmid was correctly digested and the two bands correspond to their corresponding sizes: insert~1.2kb and plasmid backbone ~ 4.9 kb.