IGEM:UNAM-Genomics Mexico/2009/Notebook/H2 Pae/2011/07/08
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Digestion of standard pBBRMCS-5
In order to see if the preffix was added correctly in the plasmid I did the digestion of the recent standard pBBRMCS-5 by EcoR1.
The digestion proportions:
The result of the digestion is shown in an electrophoresis gel (130V x 30 min):
1. Ladder 10kb 2. Helena's stuff (not important here) 3. Helena's stuff (not important here) 4. ---- 5. Standard pBBRMCS-5 digestion 6. ---- 7. Fabricio's stuff (not important here) 8. Ladder 10 kb.
It is not seen two bands in the lane 5 indicating that there is no isoforms hence correct digestion. Moreover the size (~6 kb) correspond to the sum of the pBBRMCS-5 (4950kb) plus preffix-RFP-suffix (1292 kb).