IGEM:UNAM-Genomics Mexico/2009/Notebook/H2 Pae/2011/06/28
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Results of the digestion's test
The following gel(120V x 35 min) shows the results of the pBBRMCS-5's digestion by apaI, which was made yesterday:
Lanes: 1. Ladder 10Kb. 2. Digestion. 3. pBBMRCS-5 isolation.
Here we clearly see that the isoforms in lane 3 doesn`t exit in the lane 2, besides the band corresponds to the correct size of the plasmid (arround 4.6 Kb). With this we can proceed to double digestion (apaI, sacI).
Paulina did the pBBRMCS-5's digestion only with sacI to see if it's working right. The reagents ratio is :
I made the double digestion with apaI and sacI, which will be ligated to a fragment containing prefix-RFP-suffix.
Tomorrow we'll see the results in an electrophoresis gel.