IGEM:UNAM-Genomics Mexico/2009/Notebook/H2 Pae/2011/06/18

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Digestion test(again)

Yesterday Daniel did the plasmid digestion again, but now with the correct plasmid. Today I did an electrophoresis 1% agar gel, 120 V x 35 min.

Lanes:

1.Ladder 10 kb.

2.Digestion.

3.pBBRMCS-5.


Gel 18 jul 2011.png

This shows that the plasmid was not digested because DH5-alfa cells are DCM+ so the next step is to transform the pBBRMCS-5 plasmid to other E. coli strain DCM- in this case BL21(DE3).