IGEM:UNAM-Genomics Mexico/2009/Notebook/H2 Pae/2011/06/17

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Digestion test

I did the elctrophoresis gel to verify if the digestion was successeful:

  • Lane 1 Ladder 5kb
  • Lane 2 Digestion
  • Lane 3 pBBRMCS-5

Gel 17 jun 11.JPG

With this gel I noticed that we did an incorrect digestion, because the lane 2 corresponds to the intact plasmid pS1T3 indicating that we tried to digest that palsmid, and we had to digest pBBRMCS-5. So is nedeed to repeat the digestion with the correct plasmid.