IGEM:UNAM-Genomics Mexico/2009/Notebook/H2 Pae/2011/06/06

From OpenWetWare
Jump to: navigation, search
Igem-logo-150px.png iGEM Project name 1 Report.pngMain project page
Resultset previous.pngPrevious entry      Next entryResultset next.png

Plasmid isolation kit

  • I went with Dr. Miguel Ángel Ramírez to check the kit's reagents, he said that in general (smelling them) there was nothing bad, so perhaps the problem is more specific.

Transformation

I did the tranformation of:

  • The ligation of a double digestion: From it we expect to nothing grow, because if the double digestion was correct the ends generated wouldn't be adhesives
  • The ligation of nothing (control of the ligation).
  • Nothing (only competent cells).

The transformation was made according this protocol.