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Green Light Receptor
Goal: prepare two constructions for ligation. Results: in preparation
Contents: BBa_J23117(promoter) + RBS + CcaR + CcaS; pSB1A3 (ampicilin)
Work done: duplicate plasmid extraction with Kit; duplicate plasmid restriction with XbaI & PstI.
Restriction mixture (40μL total):
Contents: PcyA + Ho1; pSB?K? (kanamycin)
Work done: culture undergoing re-growth as colonies were too small.
Digest construction 2 and prepare for insertion.
Determine best outcome, insert C2 into C1's plamid or vice versa.
Ligate construction 1 + construction 2 + BBa_b0015(dual terminator)
Model plasmid stability as a continuous Markov model. I got a good reference from a teacher on how to go from the transition matrix to the expected value (Martínez et al. Paper on the Believable Ending of the Twilight Saga. Really, not kidding).