IGEM:UNAM-Genomics Mexico/2009/Notebook/Collaborations/2010/10/12

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Chris French clarifies Yesterday's mail

Dear Luis,

sorry, didn't make myself clear - I just meant that I attached an electronic copy of the letter I sent, so that you'd know what DNA was in the package which you're waiting for.

best wishes,

Chris F.

letter for our Edinburgh shipment

Institute of Cell Biology School of Biological Sciences University of Edinburgh Darwin Building King’s Buildings Edinburgh EH9 3JR UK tel +44 (0)131 650 7098 e-mail C.French@ed.ac.uk

19 September 2010

To Whom It May Concern:

Contents: non-hazardous biological material

This letter is to certify that the contents of this package consists of non-hazardous biological material, specifically,

1-plasmid vector pSB1C3 with luxAB genes encoding bacterial luciferase. 2-plasmid vector pSB1C3 with lumP gene encoding lumazine protein. 3-plasmid vector pSB1C3 with luxAB and lumP. 4-plasmid vector pSB1C3 with luxCDE encoding substrate synthesis system for bacterial luciferase (XbaI site removed by mutation). 5-plasmid vector pSB1C3 with BioBrick insert BBa_K098101 (phycobilin synthesis genes with terminator). 6-plasmid vector pSB1C3 with BioBrick insert derived from BBa_K098010 (phycobilin synthesis genes but with terminator removed). 7-plasmid vector pSB1A3 or similar (ampR) with BioBrick insert BBa_K191004 (RFP reporter plasmid for LOV-Tap blue light sensor). 8-plasmid vector pSB1C3 with cph8 red light sensor derived from BBa_M30109 (sequence not confirmed at time of sending; will advise as soon as possible).

The plasmids are dissolved in aqueous buffer solution. This material does not contain live bacteria; it is non-toxic, non-viable, non-infectious, and poses no hazard to human health or to the environment. Please contact me at the above address if you require further information.

The intended recipient of this material is Dr. Enrique Paz of Centro de Ciencias Genómicas, Universidad Nacional Autónoma de México (tel +52 77 73 13 19 35, fax +52 77 73 17 55 81, e-mail epaz@lcg.unam.mx).

Yours truly,

Dr. Chris French Deputy Biological Safety Officer School of Biological Sciences University of Edinburgh.