IGEM:UNAM-Genomics-Mexico/2009/Notebook/iGEM 2011 UNAM-Genomics Mexico/2011/06/01

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PBBRMCS-5 Digestion Electrophoresis Gel

We run an electrophoresis gel to see the results of yesterday's digestion.

Electrophoresis Gel

We made a 1% agarose gel. (100 mL of TAE 1X and 1 gr. of agarose)

  • 1st Lane -> 5 μL DNA Ladder (Fermentas)
  • 2nd Lane -> 2.5 μL of PBBRMCS-5 digested plasmid with XBA I and Hind III, 2.5 DNA loading Dye. (1)
  • 3rd Lane -> 2.5 μL of PBBRMCS-5 digested plasmid with XBA I and Hind III, 2.5 DNA loading Dye. (2)
  • 4th Lane -> 2.5 μL of PBBRMCS-5 undigested, 2.5 DNA loading dye (control)
  • 5th Lane -> 5 μL DNA Ladder (Fermentas)

Run for 50 minutes at 110 volts.