IGEM:UNAM-Genomics-Mexico/2009/Notebook/iGEM 2011 UNAM-Genomics Mexico/2011/05/31
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PBBRMCS-5 DNA Plasmid extraction and Digestion
PBBRMCS-5 was extracted using alcaline lysis and 15μL Plasmid DNA digested with XBA I and Hind III
PBBRMCS-5 Plasmid DNA extracttion
We used Miguel Angel Ramirez's Method without the steps 11, and 13 through 20.
The gel was made with 100 mL of TAE 1X and 1 gr. of Agarose, to have a gel with 1% agarose.
The gel ran for 50 minutes at 110 volts
PBBRMCS-5 Plasmid Digestion
For a total reaction of 25 μL.
Leave all night at 37ºC in agitation.