IGEM:UBC/2009/Notebook/UBC iGEM 2010/2010/09/10

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dspB Track

Transformations

  • Protocol: SOP
    • Add 2uL of ligation mix
    • HC and HC
      • have been centrifuged down
      • took out LB broth
      • washed in water
    • HR and NR
      • still in LB broth
    • Each HC, NC, HR, NR have 2 paltes: one 50uL and one 450uL
      • 8 plates in total
    • Plates put in 37C incubator at 4:20pm