IGEM:UBC/2009/Notebook/UBC iGEM 2010/2010/09/03

iGEM Project name 1

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Biofilm Track Melody, Eric Autoclaved 30ul pipette tips Biofilm Protocol Day 4 0.1% Crystal Violet stain on plates 100831M24 + 100831M26 Biofilm Protocol Day 3 Took plate 100901M28 @ 8:06 p.m. Took plate 100901M30 @ 10:15 p.m. No contamination 3 x 300ul PBS wash of plate 100901M28 heat fixed @ 8:34 p.m. 3 x 300 ul PBS wash of plate 100901M30 heat fixed @ 10.46 p.m. dspB Track Plates taken out at 11am Lawn of colonies on all 6 plates including transformants with ccdB chlor vector. Is ccdB resistant to chloraphenicol now??? Gel verification on RD Protocol: biobrick "digest" protocol Changes: load ~17uL into each well; 10uL for ladder Gel orientation: Gel orientation ccdb chlorbb 100bp ladder RFP chlor Machine conditions: 0.5x TBE buffer, 100V, 60min Results: Gel extraction Using Invitrogen PureLink Quick Gel Extraction Kit (protocol inside kit) ccdB chlor gel weight: 0.0790g RFP chlor gel weight: 0.0878g Nanodrop: [ccdB chlor] = 5.5ng/uL [RFP chlor] = 5.2ng/uL Transformation Protocol: SOP Put in 37C incubator @ 6:47pm Plates: 1: ccdB no his 2: ccdB his 3: ccdB no his Alina 4: ccdB his Alina