IGEM:UBC/2009/Notebook/UBC iGEM 2010/2010/07/06

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dspB Track

Marianne and Vicki

Mini-prep

  • Cultures from last day taken out of turntable @ 1100 and left at room temperature on lab bench
  • Mini-prep protocol: refer to Rafael's alkaline lysis protocol
  • Mini-prep cultures from last day: 8T3, 6T10, 6T11, 6T14

Additions to protocol:

  • Place samples (8T3,6T10,6T11,6T14) in vacuum machine (Vacufuge) for 30 minutes @ 60C
  • Start: 1500
  • End: 1530
  • Measured the concentration using Nanodrop in Bolhmann's lab
  • 8T3: 395.4 ng/uL
  • 6T10: 366.3 ng/uL
  • 6T11: 349.7 ng/uL
  • 6T14: 361.5 ng/uL

Vicki Ma 18:37, 6 July 2010 (EDT)