IGEM:UBC/2009/Notebook/UBC iGEM 2010/2010/06/08
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Transformed cell cultures from yesterday (Monday) were checked at 10:00 am today. Every agar plate had colonies growing. Next step: PCR the iGEM part to verify that the colonies do contain the parts.
Competent Cell Creation See standard UBC iGEM protocol for detailed procedures. Overnight cultures of DB3.1 and DH5(alpha) were used to inoculate 2 * 50 mL cultures of each strain (11:00 am). The 50 mL cultures were grown at 30 °C. At 2:00 pm, each culture reached ~OD 0.400. We lost 1 DH5(alpha) flask due to contamination. Each 50 mL culture was split into 2 centrifuge tubes. One of the tubes was leaky, so 25 mL DB3.1 was lost.
Other (no specific track)
Tec-Monterrey iGEM Team requested E.Coli strain BW27783 which was used last year.