IGEM:The University of Sheffield/2009/Notebook/Sheffield iGEM 2010/2010/08/03

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Tuesday 3rd August:

  • BarA KO with pCAYC duet-1 transformation worked.
  • Made BarA KO competent cells.[[1]]
  • Made more glycerol stock of MG1655 and DH5alpha. ( to use for controls)
  • Made overnight culture of BarA KO with pACYC duet-1 from the successful transformation.