IGEM:Team Bordeaux/2009/Notebook/Bacterial eyespot/2012/09/17

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Day 50

Today we did the ligations IAIB , IA'IB' , ICIE , IVAIIC5B , 5AIIICIIIEIIID , 5AIVDIVEIVC , IA IIC5B , IVA5AIVB , IVEIVC with the digestions from last friday

We inactivated the ligase after 1 hour 20°C incubation and pute the tubes overnight at 4°C