IGEM:Team Bordeaux/2009/Notebook/Bacterial eyespot/2012/08/23

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Day 37

Today we did new ligations for IAIB ICIE 5AIVB IIIAIIIB IIIEIIID

We decided to now put the ligation to incubate 1 hour at 20°C then inactivate the ligase 5 min at 80°C