IGEM:Team Bordeaux/2009/Notebook/Bacterial eyespot/2012/08/08

Bacterial eyespot

Main project page Previous entry      Next entry

Day 26 Today we did a miniprep with the IAIB precultures from yesterday We got poor results from 14ng/μL to 36,6ng/μL DNA on the tubes We runned simple digested samples on an agarose gel but we couldn't discriminate the difference between the vector only and the IAIB+vector samples due to a too small deferences We'd have to run a more concentrated gel We then tried the assembly of IAIB ICIE IIAIIB IIC(1)5B We digested the bricks and tested them on agarose gel As IB digestion didn't worked we did and tested it again We then transformed the ligations into two new competent cells batches One heat shock competent DH10B stock and one heat shock competent DH5α stock We also tested the competence of the two new stocks with 1μg pBlueScript