IGEM:Slovenia HS 2015/2009/Notebook/Test/2015/07/21
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CtfA, CtfB, BdhB restriction
Restriction mixture was incubated at 37°C for 3h.
Markings: 1-10 CtfA, 11-20 CtfB, 21-30 BdhB
Agarose gel electrophoresis
Expected lenghts of genes:
Electrophoresis was successful for the restriction products. Fragments with appropriate lenghts were cut from the gel.
DNA isolation from agarose gel
po protokolu- manjka še!
These fragments were then inserted into the expression vector pSB1C3.