IGEM:Slovenia HS 2015/2009/Notebook/Test/2015/07/13

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Plasmid isolation from medium

Absorbance of constructs

To control how successful plasmid isolation was, we decided to measure absorbance of genes with nanodrop.

Control restriction

Mixture:

  • We have taken 5μL from each microcentrifuge tube
  • 0,55 ml of buffer
  • 0,2 μL of restriction enzyme Pst1

With the restriction we have linearized our plasmid.

Agarose gel electrophoresis


component quantity (µL)
oligo 1 (10µM) 1 10
oligo 2 (10µM) 1 10
dNTP (2mM) 1 10
MgCl2 2 20
buffer 2 20
dH2O 11 110