IGEM:Slovenia HS 2015/2009/Notebook/Test/2015/05/06

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DNA cloning with pure culture

Our matrix was pure Clostridium acetobutylicum culture. Two concentrations were prepeared:

  • the original concentration
  • 50x diluted

We attempted to clone the following genes:

  • 16S
  • CtfAB
  • AdhE
  • AdhE2
  • BdhA
  • BdhB

Agarose gel electrophoresis showed that only 16S was successfully cloned.