IGEM:Slovenia HS 2015/2009/Notebook/Test/2015/04/08

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DNA cloning with isolated DNA

Our matrix was DNA previously isolated from active sludge and the active sludge itself.

Two concentrations of isolated DNA were prepeared:

  • the original concentration
  • 200x diluted

We attempted to clone the following genes:

  • BdhB
  • BdhE2
  • AdhE
  • CtfAB
  • BdhA

We tried to optimise PCR by varying the annealing temperature of primers: 40, 45, 50 and 55 °C.