IGEM:Slovenia HS 2015/2009/Notebook/Test/2015/04/08
From OpenWetWare
Jump to navigationJump to search
iGEM HS Slovenia 1 | Main project page Previous entry Next entry |
DNA cloning with isolated DNAOur matrix was DNA previously isolated from active sludge and the active sludge itself. Two concentrations of isolated DNA were prepeared:
We attempted to clone the following genes:
We tried to optimise PCR by varying the annealing temperature of primers: 40, 45, 50 and 55 °C.
|