IGEM:PennState/Labbook/Nimrah Ahmed/2008/05/22

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Transformation results

None of the plates prepared yesterday grew any cells. We've started a second culture without transformation today, in a plate from the same set, to see if the problem was with our transformation procedure or (hopefully) just with the plates.

Brainstorming continues

Antibody research

  • Title: Reshaping human antibodies for therapy.
Source: Nature. 1988 Mar 24;332(6162):323-7.
Contributors: Riechmann L, Clark M, Waldmann H, Winter G.
PMID: 3127726
  • Effect of glycosylation on antibody function: implications for genetic engineering.
Trends Biotechnol. 1997 Jan;15(1):26-32.Click here to read Links
Wright A, Morrison SL.
Antibodies are able to both bind antigens and trigger the responses that eliminate them from circulation. All antibodies are glycosylated at conserved positions in their constant regions, and the presence of carbohydrate can be critical for antigen clearance functions such as complement activation. The structure of the attached carbohydrate can also affect antibody activity. Antibody glycosylation can be influenced by the cell in which it is produced, the conformation of the antibody and cell culture conditions. These variables should be considered in the design and production of antibodies with selected specificity and function.
PMID: 9032990
Link: http://www.ncbi.nlm.nih.gov/pubmed/9032990

Papers on Prokaryotic Glycosylation

  • Title: Prokaryotic glycosylation.
Source: Proteomics [1615-9853] Schäffer yr:2001 vol:1 iss:2 pg:248 -61
PMID: 11680871 [PubMed - indexed for MEDLINE]
Link: http://www3.interscience.wiley.com/cgi-bin/fulltext/77502321/PDFSTART
  • Title: Glycoproteins in prokaryotes.
Source: Arch Microbiol. 1997 Sep;168(3):169-75.
Contributors: Moens S, Vanderleyden J., F. A. Janssens Laboratory of Genetics, Catholic University of Leuven, Willem de Croylaan 42, :: B-3001 Heverlee, Belgium.

Articles/papers involving Streptomyces

  • Title: Genome sequence of an industrial microorganism Streptomyces avermitilis: Deducing the ability of producing secondary metabolites.
Source: Proc Natl Acad Sci USA 98, 12215-12220 (October 9, 2001).
Contributors: Omura, S. et al.
Link: (intro article) http://www.genomenewsnetwork.org/articles/12_01/Streptomyces_avermitilis.shtml
(main article) http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&list_uids=11572948&dopt=Abstract
PMID: 11572948

Other Project: Circuit idea : Good article a la Trevor.

Nature. 2000 Jan 20;403(6767):339-42.Click here to read Links

Construction of a genetic toggle switch in Escherichia coli.
Gardner TS, Cantor CR, Collins JJ.
Department of Biomedical Engineering, Center for BioDynamics, Boston University, Massachusetts 02215, USA.
It has been proposed' that gene-regulatory circuits with virtually any desired property can be constructed from networks of simple regulatory elements. These properties, which include multistability and oscillations, have been found in specialized gene circuits such as the bacteriophage lambda switch and the Cyanobacteria circadian oscillator. However, these behaviours have not been demonstrated in networks of non-specialized regulatory components. Here we present the construction of a genetic toggle switch-a synthetic, bistable gene-regulatory network-in Escherichia coli and provide a simple theory that predicts the conditions necessary for bistability. The toggle is constructed from any two repressible promoters arranged in a mutually inhibitory network. It is flipped between stable states using transient chemical or thermal induction and exhibits a nearly ideal switching threshold. As a practical device, the toggle switch forms a synthetic, addressable cellular memory unit and has implications for biotechnology, biocomputing and gene therapy.
PMID: 10659857 [PubMed - indexed for MEDLINE]