IGEM:PennState/Labbook/GalenLynch/2007-7-5

July 5th, 2007: 9:45-5:00

• Made new 10x dilution of SYBR green to observe rate of decay
• Ran gel testing various forms of SYBR green stock solution sample loading on 1kbp ladder
  • Dilutios: Day old 10x DMSO (gel at 1x), Day old 10x TAE (gel at 1x), Fresh 10x DMSO (gel at 1x), Day old 100x DMSO (gel at 10x)
  • results coming soon....
• transformed registry part BBa_P1010 in PSB1AK3 into DB3.1
  • Standard transformation procedure
  • Plated on amp-kan plate
• transformed ligation product of PCR for BBa_I741015 on PSB1A2 into DH5alpha
  • Standard transformation procedure
    • Probably won't work: Restriction mixture evaporated and condensed on cap of microcentrifuge tube
  • Plated on amp