IGEM:PennState/2007/Labbook/GarrettTobin/2007-8-14
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August 14th
- Miniprep xylR and B0034
- Restricted
- 1.5 hours, double digestion
- Extracted gel
- Quatnified DNA
- 5ng/ul B0034 - 16ng/ul xylR
- Ligated
- 9:9 ratio insert to vector
- 5:10 insert to vector
- (by volume)
-
- 12:30-6:30
