IGEM:Peking University/2008/Notebook/Group 3/2008/07/16
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PKU_080716_G3_1
Colony PCR-AH109+pGREG503-LacI
PCR system 15μL
Volume (μL)
buffer
5
Yeast
a few
dNTP
1.5
F-Primer
0.3
R-Primer
0.3
ddH
2
O
11.4
conditions
Temperature(°C)
Time Length(0:00:00)
98
05:00
94
00:30
35 cycles
60
00:30
35 cycles
72
02:30
35 cycles
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