IGEM:Peking University/2008/Notebook/Group 3/2008/07/16

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PKU_080716_G3_1


Colony PCR-AH109+pGREG503-LacI
File:PKU-20080716-Clony PCR-AH109+pGREG503-lacI.tif
PCR system 15μL
Volume (μL)
buffer 5
Yeast a few
dNTP 1.5
F-Primer 0.3
R-Primer 0.3
ddH2O 11.4
conditions
Temperature(°C) Time Length(0:00:00)
98 05:00
94 00:30 35 cycles
60 00:30 35 cycles
72 02:30 35 cycles