IGEM:Peking University/2008/Notebook/Group 2/2008/07/28

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PKU_080728_G2_1

Enzyme cutting test
Volume (μL)
Plasmid 5
EcoRI 0.3 ×25
HindIII 0.3
10×M Buffer 2
ddH2O 12.4




Single enzyme cutting-testing-505L site
Volume (μL)
505L 5
ApaI 0.3
10×L Buffer 2
ddH2O 12.7


Volume (μL)
505L 5
SpeI 0.3
10×L Buffer 2
ddH2O 12.7




Sequencing-513C514-No.5~No.10