IGEM:Peking University/2008/Notebook/Group 2/2008/07/25

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PKU_080725_G2_1

Frame Shifting mutation-513C514-50μL system-primers 0.75μL each


Digestion-1.4μL DpnI-37°C-2h


Self circling




Enzyme cutting-Gal4wt-ApaI-SpeI


Gel extraction


Enzyme cutting-pGREG505-lexO-ApaI-SpeI


Ethanol precipitation


Ligation-Gal4wt-pGREG505-lexO


Gel running