IGEM:Peking University/2008/Notebook/Group 2/2008/07/20

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PKU_080720_G2_1



Plasmid Miniprep-T-Gal4wt-4/T-Gal4m1278-12/T-Gal4m1743-12


Enzyme cutting-checking-T-Gal4wt/T-Gal4m1278/T-Gal4m1743
gal4(WT)-EcoRI-HindIII gal4(1278-1743)-EcoRI-HindIII(1) gal4(1278-1743)-EcoRI-HindIII(2)
File:PKU-7-20 gal4(WT)-EcoRI-HindIII.tif File:PKU-7-20 gal4(1278-1743)-EcoRI-HindIII(2).tif File:PKU-7-20 gal4(1278-1743)-EcoRI-HindIII(2).tif


Volume (μL)
plasmid 5
EcoRI 0.3 ×30
HindIII 0.3
M Buffer 2
ddH2O 12.4