IGEM:Peking University/2008/Notebook/Group 2/2008/07/01

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0701

TITLE: PCR-pPT-Gal4-Transtart 50ul*6
Volume (μL)
Template 1
dNTP 5
Enzyme 0.5
Buffer 5
F-Primer 0.5
R-Primer 0.5
ddH2O 37.5
conditions
Temperature Time Length(0:00:00)
94°C 02:00
94°C 01:00 4 cycles
50°C 02:00 4 cycles
72°C 05:00 4 cycles
94°C 01:00 30 cycles
55.2,55.6,57.6,59.5,61.3,63.9°C 01:00 30 cycles
72°C 03:00 30 cycles
72°C 10:00
4°C Hold

Enzyme cutting-T-Gal4-EcoRI-HindIII-20*4 μL
Volumn(μL)
DNA 5
EcoRI 0.2
HindIII 0.2
M Buffer 2
ddH2O 12.6

Ligation-pUC18/Gal4