IGEM:Peking University/2008/Notebook/Group 2/2008/06/05

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06-05

Enzyme cutting-pUC18-HindIII-EcoRI-20 μL
Volumn(μL)
pUC18 5
HindIII 0.2
EcoRI 0.2
M Buffer 2
ddH2O 12.6

Enzyme cutting-Gal4-HindIII-EcoRI-50 μL
Volumn(μL)
Gal4 15
HindIII 0.2
EcoRI 0.2
M Buffer 5
ddH2O 29

Ligation-pUC18/Gal4