IGEM:Peking University/2008/Notebook/Group 1/2008/08/16

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Purification PCR product-f1, f2, f3, f4, P1, P2, P3, P4

Enzyme cutting 50μL-504-AID-lexA 10μL-SacI 2μL-BamHI 0.5μL

Enzyme cutting 50μL-504-AID-lexA 20μL-BamHI 1μL-XhoI 1μL

Enzyme cutting 50μL-TSH's plasmid 10μL-BamHI 1μL-XhoI 1μL

Heat killing enzyme-65°C-20min

PCR purification-P1, P2, P3, P4, TSH, 504(4)

Gel extraction-504(2) and purification 800bp

Ligation P1-4 - 504(4)

Ligation 504(2)-TSH

Gel running and checking-ligation product
File:PKU-080816-ligation product.tif