IGEM:Peking University/2008/Notebook/Group 1/2008/07/24

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PKU_080724_G1_1

Sequencing-F-lac1-Sac-Seq

Result1: lacS-4(1.4kb), lacS-5(1.4kb), tet-7(700bp), tet-8(1.4kb); enzyme cutting sites are beyond recognization.


Sequencing-F-MQ

Result2: primer is composed wrong, a fragment from pGREG504.


Digestion-pADH-lac1/2-1/2-2/S/tet-ClaI-BamHI


Gel running and testing


Transformation-xili-lac1-1/1-2/2-1/2-2/S-1/S-2/tet-1