IGEM:Peking University/2008/Notebook/Group 1/2008/07/17

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PKU_080717_G1_1


PCR- pGREG504-pADH-(lac1/lac2/lacS/tet)-50ul
Using program lac12MQT
File:PKU-08717-PCR-pADH-promoters.tif

Enzyme cutting-pADH-(lac1/lac2/lacS/tet)(PCR product)
Volume (μL)
Promoter 15
BamHI 2
SacI 2
0.5×K Buffer 5
ddH2O 76


Volume (μL)
Promoter 15
SacI 5
XhoI 5
1×M Buffer 10
ddH2O 65



Enzyme cutting-AID
Volume (μL)
AID 15
BamHI 2
XhoI 2
1×K Buffer 10
ddH2O 71