IGEM:Peking University/2008/Notebook/Group 1/2008/07/14

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PKU_080714_G1_1


Plasmid Miniprep-pGREG504-pADH-(lac1-1/lac1-2/lac2-1/lac2-2/Control1/Control2/Control3)

Enzyme cutting-pADH-(lacS/tet)(PCR product)
Volume (μL)
pADH-(lacS/tet) 14
SacI 2
XhoI 2
M Buffer 10
ddH2O 72


Enzyme cutting-pGREG504-1
Volume (μL)
pGREG504-1 4
SacI 2
XhoI 2
M Buffer 10
ddH2O 82


File:PKU 080714 lacS-tet-pGREG504cut.tif
lanes:lacS|tet|vector|marker1|marker2


Enzyme cutting-pGREG504-pADH-(lac1-1/lac1-2/lac2-1/lac2-2/Control1/Control2/Control3)
Volume (μL)
pGREG504-pADH-(lac1-1/lac1-2/lac2-1/lac2-2/Control1/Control2/Control3) 5
SacI 1
XhoI 1
M Buffer 2
ddH2O 11


Volume (μL)
pGREG504-pADH-(lac1-1/lac1-2/lac2-1/lac2-2) 5
SphI 0.2
XhoI 0.2
H Buffer 2
ddH2O 12.6


Volume (μL)
pGREG504-pADH-(Control1/Control2/Control3) 5
BamHI 0.2
XhoI 0.2
K Buffer 2
ddH2O 12.6