IGEM:Peking University/2008/Notebook/Group 1/2008/05/19

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PKU_080519_G1_1


Cultivation- E.Coli(liguid-Amp+)

Plasmid Miniprep-pGREG504/pADH

Enzyme cutting-pGREG504/pADH-EcoRV
File:PKU 080519 check-pGREG504-pADH.tif
Volume (μL)
pGREG504/pADH 5
EcoRV 0.2 *13
H Buffer 2
ddH2O 12.8


Enzyme cutting-pGREG504/pADH-SacI-BamHI
Volume (μL)
pGREG504/pADH 5
SacI 0.2 *13
BamHI 0.2
K Buffer 2
ddH2O 13.6