IGEM:Peking University/2008/Notebook/Group 1/2008/05/14

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PKU_080514_G1_1


PCR- pGREG505/(TEF-lacI-ADHlacS-GFP)-adhlac-50ul
File:PKU 080514 ADH-PCR-check.tif
Volume (μL)
pGREG505/(TEF-lacI-ADHlacS-GFP) 1
easytaq mixture 25 *3
F-Primer 0.5
R-Primer 0.5
ddH2O 23


conditions
Temperature(°C) Time Length(0:00:00)
94 05:00
94 00:30 30 cycles
50.1,52.2,55.1,58.3,62.2 01:00 30 cycles
72 01:30 30 cycles
72 07:00
Alarm 5 times
Hold 4°C