IGEM:Paris Liliane Bettencourt/Notebook/Paris 2010/2010/08/23

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Raphaël
Minipreps

  • Ps/sRBS/LuxR/attC/t/attC/mRFP
  • Ps/mRBS/LuxR/attC/t/attC/mRFP
  • Ps/wRBS/LuxR/attC/t/attC/mRFP


Restriction digest with SpeI and PstI : 20µl miniprep + 2.5µl B2 + 2x1µl enzymes + 0.25µl BSA

  • Ps/sRBS/LuxR/attC/t/attC/mRFP
  • Ps/mRBS/LuxR/attC/t/attC/mRFP
  • Ps/wRBS/LuxR/attC/t/attC/mRFP


Gel electrophoresis

  • Ps/wRBS/LuxR/attC/t/attC/mRFP
  • -
  • Ps/mRBS/LuxR/attC/t/attC/mRFP
  • -
  • Ps/sRBS/LuxR/attC/t/attC/mRFP
  • Ladder 1kb

File:2308 1.jpg


Gel extraction

  • Ps/sRBS/LuxR/attC/t/attC/mRFP
  • Ps/mRBS/LuxR/attC/t/attC/mRFP

Aleksandra
PCR, 1µl miniprep (13/08) + 2x1µl primers 0.025ng/µl + 10µl water + 13µl 2x quick load mix, Tm=60, Telong=68

  • wRBS/TetA(C)
  • mRBS/TetA(C)
  • sRBS/TetA(C)


Gel electrophoresis

  • wRBS/TetA(C)
  • mRBS/TetA(C)
  • sRBS/TetA(C)
  • Ladder 1Kb

File:2308 2.jpg
-> bands of agarose in the fridge