IGEM:Paris Liliane Bettencourt/Notebook/Paris 2010/2010/08/19

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Léa Restriction digest with XbaI and PstI : 5µl miniprep + 15µL water + 2x1µl enzyme + 2.5µl B3 + 0.25µl BSA 3x Ps/wRBS/LuxR + wRBS/TetA(C)/LuxI 4x Ps/wRBS/LuxR + mRBS/TetA(C)/LuxI 1x Ps/sRBS/LuxR + mRBS/TetA(C)/LuxI 1x Pm/sRBS/LuxR + sRBS/TetA(C)/LuxI 2x Ps/wRBS/LuxR + sRBS/TetA(C)/LuxI 2x Ps/mRBS/LuxR + sRBS/TetA(C)/LuxI 3x pLux/LuxI Gel electrophoresis Ps/wRBS/LuxR + wRBS/TetA(C)/LuxI, colony II Ps/wRBS/LuxR + wRBS/TetA(C)/LuxI, colony III Ps/wRBS/LuxR + wRBS/TetA(C)/LuxI, colony IV Ps/wRBS/LuxR + mRBS/TetA(C)/LuxI, colony I Ps/wRBS/LuxR + mRBS/TetA(C)/LuxI, colony II Ps/wRBS/LuxR + mRBS/TetA(C)/LuxI, colony III Ps/wRBS/LuxR + mRBS/TetA(C)/LuxI, colony IV Ps/sRBS/LuxR + mRBS/TetA(C)/LuxI, colony I Pm/sRBS/LuxR + sRBS/TetA(C)/LuxI, colony I Ps/wRBS/LuxR + sRBS/TetA(C)/LuxI, colony I Ps/wRBS/LuxR + sRBS/TetA(C)/LuxI, colony II Ps/mRBS/LuxR + sRBS/TetA(C)/LuxI, colony I Ps/mRBS/LuxR + sRBS/TetA(C)/LuxI, colony IV pLux/LuxI, colony I pLux/LuxI, colony II pLux/LuxI, colony III Ladder 1kb File:Gel1908 1.jpg Aleksandra Overnight cultures Ps/wRBS/LuxR + attC/term/attC/mRFP Ps/mRBS/LuxR + attC/term/attC/mRFP Ps/sRBS/LuxR + attC/term/attC/mRFP Pm/sRBS/LuxR + attC/term/attC/mRFP pLux wRBS/LacZ sRBS/LacZ wRBS/TetA(C) mRBS/TetA(C) sRBS/TetA(C) 2x RBS/LuxI