IGEM:Paris Liliane Bettencourt/Notebook/Paris 2010/2010/08/19

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Léa
Restriction digest with XbaI and PstI : 5µl miniprep + 15µL water + 2x1µl enzyme + 2.5µl B3 + 0.25µl BSA

  • 3x Ps/wRBS/LuxR + wRBS/TetA(C)/LuxI
  • 4x Ps/wRBS/LuxR + mRBS/TetA(C)/LuxI
  • 1x Ps/sRBS/LuxR + mRBS/TetA(C)/LuxI
  • 1x Pm/sRBS/LuxR + sRBS/TetA(C)/LuxI
  • 2x Ps/wRBS/LuxR + sRBS/TetA(C)/LuxI
  • 2x Ps/mRBS/LuxR + sRBS/TetA(C)/LuxI
  • 3x pLux/LuxI


Gel electrophoresis

  • Ps/wRBS/LuxR + wRBS/TetA(C)/LuxI, colony II
  • Ps/wRBS/LuxR + wRBS/TetA(C)/LuxI, colony III
  • Ps/wRBS/LuxR + wRBS/TetA(C)/LuxI, colony IV
  • Ps/wRBS/LuxR + mRBS/TetA(C)/LuxI, colony I
  • Ps/wRBS/LuxR + mRBS/TetA(C)/LuxI, colony II
  • Ps/wRBS/LuxR + mRBS/TetA(C)/LuxI, colony III
  • Ps/wRBS/LuxR + mRBS/TetA(C)/LuxI, colony IV
  • Ps/sRBS/LuxR + mRBS/TetA(C)/LuxI, colony I
  • Pm/sRBS/LuxR + sRBS/TetA(C)/LuxI, colony I
  • Ps/wRBS/LuxR + sRBS/TetA(C)/LuxI, colony I
  • Ps/wRBS/LuxR + sRBS/TetA(C)/LuxI, colony II
  • Ps/mRBS/LuxR + sRBS/TetA(C)/LuxI, colony I
  • Ps/mRBS/LuxR + sRBS/TetA(C)/LuxI, colony IV
  • pLux/LuxI, colony I
  • pLux/LuxI, colony II
  • pLux/LuxI, colony III
  • Ladder 1kb


File:Gel1908 1.jpg


Aleksandra


Overnight cultures

  • Ps/wRBS/LuxR + attC/term/attC/mRFP
  • Ps/mRBS/LuxR + attC/term/attC/mRFP
  • Ps/sRBS/LuxR + attC/term/attC/mRFP
  • Pm/sRBS/LuxR + attC/term/attC/mRFP
  • pLux
  • wRBS/LacZ
  • sRBS/LacZ
  • wRBS/TetA(C)
  • mRBS/TetA(C)
  • sRBS/TetA(C)
  • 2x RBS/LuxI