IGEM:Paris Liliane Bettencourt/Notebook/Paris 2010/2010/08/12

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Raphaël and Aleksandra
Glycerols, 15%

  • mRBS/TetA(C)+RBS/LuxI, 4 colonies
  • wRBS/TetA(C)+RBS/LuxI, 4 colonies
  • stRBS/TetA(C)+RBS/LuxI, 4 colonies
  • RBS/mRFP/t(K081014)+pLux/GFP, 4 colonies
  • RBS/mRFP/tt(I13507)+pLux/GFP, 4 colonies
  • Ps/wRBS/LuxR +attC/term/attC/mRFP, 3 colonies
  • Ps/mRBS/LuxR +attC/term/attC/mRFP, 4 colonies
  • Pm/sRBS/LuxR +attC/term/attC/mRFP, 1 colony
  • Ps/sRBS/LuxR +attC/term/attC/mRFP, 4 colonies


Léa and Théotime
Minipreps of 10ml, elution in 35µl warm water

  • mRBS/TetA(C)+RBS/LuxI, 4 colonies
  • wRBS/TetA(C)+RBS/LuxI, 4 colonies
  • stRBS/TetA(C)+RBS/LuxI, 4 colonies
  • RBS/mRFP/t(K081014)+pLux/GFP, 4 colonies
  • RBS/mRFP/tt(I13507)+pLux/GFP, 4 colonies
  • Ps/wRBS/LuxR +attC/term/attC/mRFP, 3 colonies
  • Ps/mRBS/LuxR +attC/term/attC/mRFP, 4 colonies
  • Pm/sRBS/LuxR +attC/term/attC/mRFP, 1 colony
  • Ps/sRBS/LuxR +attC/term/attC/mRFP, 4 colonies


Restriction digest

  • mRBS/TetA(C)+RBS/LuxI, 4 colonies with EcoRI-HF and PstI : 5µl miniprep + 2µl B2 + 0.2µl BSA + 12µl water + 2x1µl enzymes
  • wRBS/TetA(C)+RBS/LuxI, 4 colonies with EcoRI-HF and PstI : 5µl miniprep + 2µl B2 + 0.2µl BSA + 12µl water + 2x1µl enzymes
  • stRBS/TetA(C)+RBS/LuxI, 4 colonies with EcoRI-HF and PstI : 5µl miniprep + 2µl B2 + 0.2µl BSA + 12µl water + 2x1µl enzymes
  • RBS/mRFP/t(K081014)+pLux/GFP, 4 colonies with EcoRI-HF and PstI : 5µl miniprep + 2µl B2 + 0.2µl BSA + 12µl water + 2x1µl enzymes
  • RBS/mRFP/tt(I13507)+pLux/GFP, 4 colonies with EcoRI-HF and PstI : 5µl miniprep + 2µl B2 + 0.2µl BSA + 12µl water + 2x1µl enzymes
  • Ps/wRBS/LuxR +attC/term/attC/mRFP, 3 colonies with SpeI and PstI : 5µl miniprep + 2µl B2 + 0.2µl BSA + 12µl water + 2x1µl enzymes
  • Ps/mRBS/LuxR +attC/term/attC/mRFP, 4 colonies with SpeI and PstI : 5µl miniprep + 2µl B2 + 0.2µl BSA + 12µl water + 2x1µl enzymes
  • Pm/sRBS/LuxR +attC/term/attC/mRFP, 1 colony with SpeI and PstI : 5µl miniprep + 2µl B2 + 0.2µl BSA + 12µl water + 2x1µl enzymes
  • Ps/sRBS/LuxR +attC/term/attC/mRFP, 4 colonies with SpeI and PstI : 5µl miniprep + 2µl B2 + 0.2µl BSA + 12µl water + 2x1µl enzymes


Gel electrophoresis, 1.5%agarose, for verification only
Gel 1

  • mRBS/TetA(C)+RBS/LuxI col1
  • mRBS/TetA(C)+RBS/LuxI col2
  • mRBS/TetA(C)+RBS/LuxI col3
  • mRBS/TetA(C)+RBS/LuxI col4
  • wRBS/TetA(C)+RBS/LuxI col1
  • wRBS/TetA(C)+RBS/LuxI col2
  • wRBS/TetA(C)+RBS/LuxI col3
  • wRBS/TetA(C)+RBS/LuxI col4
  • stRBS/TetA(C)+RBS/LuxI col1
  • stRBS/TetA(C)+RBS/LuxI col2
  • stRBS/TetA(C)+RBS/LuxI col3
  • stRBS/TetA(C)+RBS/LuxI col4
  • RBS/mRFP/t(K081014)+pLux/GFP col1
  • RBS/mRFP/t(K081014)+pLux/GFP col2
  • RBS/mRFP/t(K081014)+pLux/GFP col3
  • RBS/mRFP/t(K081014)+pLux/GFP col4

File:Gel1208 1.jpg


Gel 2

  • RBS/mRFP/tt(I13507)+pLux/GFP col1
  • RBS/mRFP/tt(I13507)+pLux/GFP col2
  • RBS/mRFP/tt(I13507)+pLux/GFP col3
  • RBS/mRFP/tt(I13507)+pLux/GFP col4
  • Ps/wRBS/LuxR +attC/term/attC/mRFP col1
  • Ps/wRBS/LuxR +attC/term/attC/mRFP col2
  • Ps/wRBS/LuxR +attC/term/attC/mRFP col3
  • Ps/mRBS/LuxR +attC/term/attC/mRFP col1
  • Ps/mRBS/LuxR +attC/term/attC/mRFP col2
  • Ps/mRBS/LuxR +attC/term/attC/mRFP col3
  • Ps/mRBS/LuxR +attC/term/attC/mRFP col4
  • Pm/sRBS/LuxR +attC/term/attC/mRFP
  • Ps/sRBS/LuxR +attC/term/attC/mRFP col1
  • Ps/sRBS/LuxR +attC/term/attC/mRFP col2
  • Ps/sRBS/LuxR +attC/term/attC/mRFP col3
  • Ps/sRBS/LuxR +attC/term/attC/mRFP col4