IGEM:Paris Liliane Bettencourt/Notebook/Paris 2010/2010/08/06

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Aleksandra
Transformation of the TURBO cells with yesterday's ligations (150µl per transformation)

  • Pmed + mRFP
  • Pst + mRFP
  • Pmed/strRBS/LuxR + attC/term/attC/mRFP
  • Pmed/medRBS/LuxR + attC/term/attC/mRFP
  • Pmed/weakRBS/LuxR + attC/term/attC/mRFP
  • Pstr/weakRBS/LuxR + attC/term/attC/mRFP
  • Pstr/medRBS/LuxR + attC/term/attC/mRFP
  • Pstr/strRBS/LuxR + attC/term/attC/mRFP
  • Ps ctrl
  • Pm ctrl
  • Pm/weakRBS/LuxR ctrl
  • Psstr/strRBS/LuxR ctrl


Raphaël
Minipreps
Elution in 60µl water

  • Pm/weakRBS/LuxR
  • Ps/weakRBS/LuxR
  • Pm/medRBS/LuxR
  • Ps/medRBS/LuxR
  • Pm/strRBS/LuxR
  • Ps/strRBS/LuxR


Elution in 30µl water

  • weakRBS/TetA(C)
  • medRBS/TetA(C)
  • strRBS/TetA(C)
  • weakRBS/LacZα
  • strRBS/LacZα
  • 11 minipreps of attC/term/attC/mRFP


Théotime
Overnight Culture in 12mL LB AMP.

  • Pm/weakRBS/LuxR/attC/term/attC/mRFP (x3)
  • Ps/weakRBS/LuxR/attC/term/attC/mRFP (x2)
  • Pm/medRBS/LuxR/attC/term/attC/mRFP (x1)
  • Ps/medRBS/LuxR/attC/term/attC/mRFP (x2)
  • Pm/stdRBS/LuxR/attC/term/attC/mRFP (x3)
  • Ps/stdRBS/LuxR/attC/term/attC/mRFP (x1)
  • Ps/RFP (x4)