IGEM:Paris Liliane Bettencourt/Notebook/Paris 2010/2010/07/17

From OpenWetWare
Jump to: navigation, search
Igem-logo-150px.png Population counter Report.pngMain project page
Resultset previous.pngPrevious entry      Next entryResultset next.png


Aleksandra
Minipreps of the overnight cultures (Qiagen kit)
2 series of minipreps of 5 ml culture each :

  • attC - Kan
  • term-attC-mRFP - Kan
  • term (B0015) - Amp
  • RBS/GFP/term (E0240) - Amp
  • RBS/LuxR (J37033) - Amp
  • pLux (R0062) - Amp
  • RBS/LuxI (K081008) - Amp
  • Pstrong (J23119) - Amp
  • Pmedium (J23110) - Amp


DNA from both minipreps is collected into the same tube in the end -> 2x30 µL = 60 µL DNA of each plasmid in the end