IGEM:PKU Beijing/2009/Notebook/AND Gate 1/Input/2009/07/11/Rencheng Gao

From OpenWetWare
Jump to: navigation, search

11:00
Store the strains of and mini-prep: 1-11N+AraC(2), 1-5J+AraC(2), 1-1H+AraC(2), 1-2M+AraC(2), 1-5N+AraC(3), 1-2I+AraC(2)
BioSpec(×50 μg/mL):

1-5J 2.8885
1-5N 3.7763
1-2I 3.8228
1-11N 5.0811
1-1H 4.0099
1-2M 3.7531

16:20
Double-enzyme digestion : 1-11N, 1-1H, 1-2M

22:30
DNA purification: fragments of 1-11N, 1-1H, 1-2M

00:30
Ligation
Insert: fragments of 1-11N, 1-1H, 1-2M
Vector: 1-23L