IGEM:PKU Beijing/2009/Notebook/AND Gate 1/Input/2009/07/09/Hao Wang
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9:06 Miniprep the 2-4O->1-18P plasmids
11:52 Digest the plasmids of 2-4O->1-18P and 1-23L:
Digestion System Insert: 20μL;
| Plasmids | 5μL |
| EcoR1 | 1μL |
| Spe1 | 1ul |
| Buffer 1*H | 2μL |
| dd H2O | 11μL |
Digestion System Vector: 20μL;
| Plasmids | 5μL |
| EcoR1 | 1μL |
| Xbal1 | 1ul |
| Buffer 1*M | 2μL |
| dd H2O | 11μL |
12:13 Reaction Start
17:32 Stop the digestion
20:08 Prepare to Ligation
20:17 Ligation start
