IGEM:PKU Beijing/2009/Notebook/AND Gate 1/Core/2009/07/14/Zhangs

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Electrophoresis and Gel Extraction for T7ptag+1-23L insert
Result (from left to right)
T7ptag①, T7ptag②, Marker
PKU 20090714 Zhangs 1.JPG
Gel: T7ptag+1-23L 0.125gPN volume: 375μL

Ligate the T7ptag+1-23L insert and 9 RBS vector
System

3μL insert
1μL RBS vector
1μL 10× Ligase buffer
1μL Ligase
4μL ddH2O
10μL Total