IGEM:PKU Beijing/2009/Notebook/Bistable/2009/07/21/Shan Shen

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11:00
PCR the clones of high-copy bi-stable system to test if they are correct.

13:30
Electrophoresis to test the PCR products.
The order and the amount of the samples: marker 5μL, bi-stable 1, bi-stable 2, bi-stable 3.
Results:
PKU 20090721 Shan Shen 1.JPG
All of the three clones are incorrect according to the results of PCR.

14:21
Miniprep bi-stable 1,2,3,SupD1 and 1-9H.
Concentrations:

Number of the plasmids Concentration(ng/μL)
Bi-stable 1 160
Bi-stable 2 200
Bi-stable 3 250
SupD1 250
1-9H 110

16:50
Digest the bi-stable system to test whether the ligations are correct

Total 10μL
Plasmids 1μL
EcoR1 0.5μL
Pst1 0.5μL
Buffer 1μL
ddH2O 7μL

17:15
Start to digest.

20:00
Electrophoresis to test the digestion products.
The order and the amount of the samples: marker 5μL, bi-stable plasmids1, bi-stable 1 digestion products, bi-stable 2 plasmids, bi-stable 2 digestion products, bi-stable plasmids 3, bi-stable 3 digestion products.
Results:
PKU 20090721 Shan Shen 2.JPG