IGEM:PKU Beijing/2009/Notebook/Bistable/2009/07/14/Shan Shen

From OpenWetWare
Jump to: navigation, search

12:30
PCR to test GFP

15:30
Electrophoresis to test the PCR products.
The order and the amount of the samples: marker 5μL, clone 1 10μL, clone 2 10μL, clone 3 10μL, clone 4 10μL, clone 5 10μL.
PKU 20090714 Shan Shen 1.JPG
The band appeared at about 1kb seems to be the correct products.

16:00
PCR bi-stable to insert into the chromosome.

Total 20μL
Template 1μL
dNTP 4μL
Buffer 2μL
Easypfu 1μL
For-primer 1μL
Rev-primer 1μL
ddH2O 10μL

20:30
Electrophoresis to test the PCR products.
The order and the amount of the samples: marker 5μL, bi—stable PCR products, plasmid control of low-copy bi-stable system, digestion products of low-copy bi-stable system.
Results:
PCR and digestion are both unsuccessful.