IGEM:PKU Beijing/2009/Notebook/Bistable/2009/07/14/Min Lin
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16:00
MiniPrep low copy plasmid of the bistable plasmid.
Concentration = 130ng/ul
Enzyme Digestion with
| EcoRI | 1ul |
| PstI | 1ul |
| 10xH buffer | 2ul |
| Plasmid | 7ul |
| ddH2O 9ul |
GEL:
Low copy bistable plasmid is lost.
Ask Louchunbo about it, the low copy plasmid is not standarlized.
