IGEM:PKU Beijing/2009/Notebook/Bistable/2009/07/13/Shan Shen
Electrophoresis to test the digestion products of 1-18A
The order and the amount of the samples: marker 5μL, plasmids of 1-18A 2μL, digestion products of 1-18A 20μL.
The digestion products still run slower than the uncut plasmids, but 1-18A is tested to be gel-bad according to the registry, so that the products are probably correct.
Continue to prepare competent cells.
Ligation of the 1-18A vector and 1-12O (GFP) inserts.
Transformation of the ligation products and 1-1D, 1-6I to test the efficiency of the competent cells.