IGEM:PKU Beijing/2009/Notebook/Bistable/2009/07/08/Shan Shen
From OpenWetWare
Jump to navigationJump to search
9:00 Pick JM109 into LB without any resistance for incubation (37゚C)
10:00 Pick 1-18A, 1-18C into LB with Amp for incubation.
15:00 Prepare SOB.
17:00 Pick bi-stable cells of high-copy and low-copy to incubate.
22:10 Miniprep the plasmids of 1-18A and 1-18C
Number of the plasmids | Concentration(ng/μL) |
1-18A | 270 |
1-18C | 167 |
23:10 Double digestion of 1-18A.
Total | 20μL |
Plasmids | 4μL |
Spe1 | 1μL |
Pst1 | 1μL |
Buffer | 2μL |
ddH2O | 12μL |